FOLLICULAR ASPIRATION TECHNIQUES
OPU TRAINING, THEORY AND PRACTICAL COURSE
OVOLEARNING the training platform of OVOHORSE & OVOCLONE offers a program to train veterinarians in the OPU technique.
OPU or Oocyte Collection: is the initial process to carry out the equine assisted reproduction treatment. During this first phase, the oocytes (eggs) are extracted from the ovary using the follicular aspiration technique which is guided by transvaginal ultrasound.
Programme
THEORY AND PRACTICAL COURSE
The course is divided into a theoretical and practical module where we will study the physiology of the mare’s oocyte, in order to understand the importance of proper handling of the oocyte when it is removed from the reproductive tract and taken to the laboratory. In addition, we will go into the different techniques available to obtain oocytes describing the advantages and disadvantages of each one, including all the technical details and laboratory protocols. Hands-on training, participants will have the possibility to perform their own follicular aspiration and search for their own oocytes.
| 09:00 – 09:15 | Opening of the course. Dr. Andrés Gambini and Enrique Criado. |
| 09:15 – 10:30 | Physiology of the mare’s ovary. Practical training for follicular follicular aspiratio/td> |
| 10:30 – 10:45 | Break |
| 10:45 – 12:30 | Equine oocyte physiology. Practical training in the handling of the oocyte in the laboratory. |
| 12:30 – 14:30 | Lunch |
| 14:30 – 19:00 | Methods of obtaining oocytes and handling of the oocyte in the laboratory. Practical: searching for and handling of oocytes (mare ovaries). Holding media, culture, maturation, oocyte. |
| 08:00 – 09:30 | Follicular aspiration theory. |
| 10:00 – 12:00 | Practical demonstration of follicular aspiration. |
| 12:00 – 13:00 | Lunch |
| 13:00 -17:00 | Practical follicular aspiration |
| 17:00 – 17:30 | Coffee break |
| 17:30 – 19:30 | Oocyte Search |
GROUP 1 |
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| 08:00 – 13:00 | OPU Practice | ||
| 13:00 – 15:00 | Lunch | ||
| 15:00 – 20:00 | OPU Practice | ||
GROUP 2 |
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| 08:00 – 13:00 | Lab tour | ||
| 13:00 – 15:00 | Lunch | ||
| 15:00 – 20:00 | Table discussion – Problem Solving | ||
Target group: veterinarians.
Maximum number of places: limited.
Dates: February and March PLACES SOLD OUT.
Next course: April.
Place: Marbella.
Training content:
Follicular aspiration technique, risk assessment.
Care before, during and after OPU.
Administration of medications before, during and after OPU.
Preparation of samples for oocyte shipment.
SPEAKERS
Enrique Criado Scholz
CEO of Ovoclinic, Ovobank, Ovohorse & Ovoclone group. Embryologist trained in the latest laboratory techniques.
Andrés Gambini
Scientific Director of Ovohorse. Specialist in animal cloning and biotechnology.
INSTALLATIONS
UPCOMING COURSES
OPU TRAINING, THEORY AND PRACTICAL COURSE
FEBRUARY
PLACES SOLD OUT
MARCH
PLACES SOLD OUT
APRIL
1 – 3
MAY
6 – 8
JUN
3 – 5
